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6624 ag  (R&D Systems)


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    Structured Review

    R&D Systems 6624 ag
    6624 Ag, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+protein+recombinant+human+agrin+protein/Recombinant+Human+Agrin+Protein%2C+CF/us12576191-141-9-6
    Average 94 stars, based on 24 article reviews
    6624 ag - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Recombinant:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Purification:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Binding Assay:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Produced:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Genome Wide:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Software:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Functional Assay:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.

    Flow Cytometry:

    Article Title: Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
    Article Snippet: DOI: https://doi.org/10.7554/eLife.46840 28 of 43 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti rat IgG Alexa 546 (goat polyclonal) Invitrogen #A-11081; RRID:AB_141738 (1:300); used for IF staining of BM Antibody anti-actin (mouse monoclonal) Sigma-Aldrich #A4700, clone AC-40; RRID:AB_476730 (1:1000) Antibody Anti-a-tubulin (mouse monoclonal) Sigma-Aldrich #T6199, clone DM1A; RRID:AB_477583 (1:1000) Antibody anti-GAPDH (rabbit monoclonal) Cell Signaling Technology #2118, clone: 14C10; RRID:AB_561053 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Src p-Tyr418 (rabbit polyclonal) Sigma-Aldrich #44660G; RRID:AB_1500523 (1:10) dilution, on 0.05 mg/ml lysates for Wes Antibody anti-Shp1 p-Tyr564 (rabbit monoclonal) Cell Signaling Technology #8849, clone: D11G5; RRID:AB_11141050 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr542 (rabbit polyclonal) Cell Signaling Technology #3751; RRID:AB_330825 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Shp2 p-Tyr580 (rabbit polyclonal) Cell Signaling Technology #3703; RRID:AB_2174962 (1:10) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-Syk p-Tyr525/6 (rabbit polyclonal) Cell Signaling Technology #2711; RRID:AB_2197215 (1:50) dilution, on 0.2 mg/ml lysates for Wes Antibody anti-SH-PTP1/Shp-1 (rabbit polyclonal) Santa Cruz sc-287 (C19); RRID:AB_2173829 (1:1000) Antibody anti-SH-PTP2/Shp-2 (rabbit polyclonal) Santa Cruz sc-280 (C18); RRID:AB_632401 (1:1000) Antibody anti-phosphotyrosine (mouse monoclonal) Merck-Millipore 05–321, clone 4G10; RRID:AB_309678 (1:1000) Antibody anti-human G6b-B (mouse monoclonal) other clone 17–4 10 mg/ml, custom-made lab reagent Peptide, recombinant protein purified human IgG-Fc fragment Bethyl Laboratories P80-104 Peptide, recombinant protein recombinant Mouse Syndecan-2/CD362 protein, CF R&D Systems 6585-SD-050 Peptide, recombinant protein recombinant human Agrin protein, N-terminal, CF R&D Systems 8909-AG-050 Peptide, recombinant protein rec. human laminin 111 Biolamina LN111-02 Peptide, recombinant protein rec. human laminin 411 Biolamina LN411-02 Peptide, recombinant protein rec. human laminin 421 Biolamina LN421-02 Peptide, recombinant protein rec. human laminin 511 Biolamina LN511-02 Continued on next page Vögtle et al. eLife 2019;8:e46840.



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